Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKVERLLINYKTLEEFKKFKEYGIQELSMLEELQDNIIENDSTSPFYGIYFGDKLVARMSLYQVNGKSN------PYFDNRQDYLELWKLEVLPGYQNRGYGRALVEFAKSFK----MPIRTNPRMK-SAEFWNKMNFKTVKYDMARDKGENPLIWHPDMDREMTPGESA 2PC1 Chain:A ((71-173)) --------------------------------------------GYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISNQFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPL------------------------

General information: TITO was launched using: RESULT: Template: 2PC1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 317 -58485 -184.49 -635.70 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -184.49 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.436

(partial model without unconserved sides chains): PDB file : Tito_2PC1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PC1-query.scw PDB file : Tito_Scwrl_2PC1.pdb: