Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFQHKTVLLRETVDGLNIKPDGTYVDCTLGGAGHSTYLLQQLSEKGRLIAFDQDDTALQHAKEVLSD--YKGQLILIKSNFRYLKEYLNEQGVTEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQSATLSAKEVVNEWRYEDLVRIFFKYGEEKFSKQIARKIEEARMKSPIQTTGQLVDLIKDAIPAP-----ARRSGGHPAKRVFQAIRIAVNDELRVFEEALEQAIEVLKPGGRVSVITFHSLEDRICKTTFKEKSSLPELPPGLPVIPEEFEPELKLITRKPITASQEELEENNRARSAKLRIAEKRK 3EEY Chain:A ((3-197)) LTIKNSLGQSHDYIKMFVKEGDTVVDATCGNGNDTAFLASLVGENGRVFGFDIQDKAIANTTKKLTDLNLIDRVTLIKDGHQNMDKYIDCP----VKAVMFNLG----YLPSGDHSISTR---------PETTIQALSKAMELLVTGGIITVVIYYGGDTGFEEKEKVLEFLKGVDQKKFIVQRTDFINQANCPPILVCIEKISEGHHHHHH----------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3EEY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 911 -66177 -72.64 -352.01 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -72.64 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_3EEY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EEY-query.scw PDB file : Tito_Scwrl_3EEY.pdb: