Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------MLVYI-GNGHYCYSNSTAMFLSSIGENVSPQLVEILTGVGLGA-MIEYEKNLYF-SMRDPDDGINY-ALNILGFTAEE-HQQASDLDDPFPLLKQQIKQNPVILGPLDMGELT--YHPNHKNLNGSDHYVLGYQMDNENIYVQDPAGFPFVPLSLDQFKKAWMAERIPYRKGINKYWSTAKKVVTL-DNNEIYERAIDYFKRTYRKFEKVDIGLIGREAICFYADQLLNAPITADTIRHTTFFLFQLSARRANDYAMYFKDRHSHLSVLKTEQAKVFGICHSMSVNKDWKGISEKLMKLADLEDNFR-LELLKVGY 3RHT Chain:A ((6-257)) RVLYCGDTSLETAAGYLAGLMTSWQWEFDYIPSHVGLDVGELLAKQDLVILSDYPAERMTAQAIDQLVTMV---KAGCGLVMLGGWESYHGLGGNWDQTLLAEVLPVD---------IKSADDRINFDQPTLAIPAAINSVSHPIL---------------------------QNLPWEDRPPTIGGLNRIAAKAKAQTLLMARVWRPTFSLEHGKTTWEHADHHPLLVVGEA------GTGRVAAFASDVAPH-----WVGGLVDWGDERVTSQAPGAGAIEVGNLYSQFFR----------------------------QMLEWVAKS-

General information: TITO was launched using: RESULT: Template: 3RHT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1244 20033 16.10 87.86 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 16.10 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_3RHT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RHT-query.scw PDB file : Tito_Scwrl_3RHT.pdb: