Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRD---MKKEECVRLISHFKFLSAMDIGERRKPQNGSLTLKLKE-GNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQYAKKHFNRNIVTLEDPVETRDED----VLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLHTRDAKGAIYRLLEFGIN------MNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYG-KNLQQCIQEAKGN---------HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD 2EWV Chain:A ((12-357)) ----ELKILEIIKEAIELGASDIHLTA---GAPPAVRIDGYIKFLKDFPRLTPEDTQKLAYSVMSEK----HRQKLEENGQVDFSFGVRGVGRFRANVFYQRGSVAAALRSLPAEIP-EFKKLGLPDKV----LELCHRKMGLILVTGPTGSGKSTTIASMIDYINQTKSYHIITIEDPIEYVFKHKKSIVNQREVG-EDTKSFADALRAALREDPDVIFVGEMRDLETVETALRAAETGHLVFGTLHTNTAIDTIHRIVDIFPLNQQEQVRIVLSFILQGIISQRLLPKIGG-----------------GRVLAYELLIPNTAIRNL--IRENKLQQVYSLMQ-----MQTMNQTLYKLYKQGLITLEDAMEASPDPKE

General information: TITO was launched using: RESULT: Template: 2EWV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1607 23901 14.87 75.87 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 14.87 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.418

(partial model without unconserved sides chains): PDB file : Tito_2EWV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EWV-query.scw PDB file : Tito_Scwrl_2EWV.pdb: