Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSQLRKKTLEALSALSNEDILQKTERMYKYLFSLPEWQNAGTIAVTISR--GLEIPTRPVIEQAWE-----------EGKQVCIPKCHPDTKKMQFRTYQTDDQLETVYA---------------GLLEPVIEKTKEVNPSQIDLMIVPGVCFDVNGFRVGFGGGYYDRYLSEYEG-KTVSLLLECQLFAHVPRLPHDIPVHKLITE-----DRIISCFS 2VQ2 Chain:A ((4-223)) ANQVSNIKTQLAMEYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPDSAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLAEAYLKRSLAAQPQFPPAFKELARTKMLAG-QLGDADYYFKKYQSRVEVLQADDLLLGWKIAKALGNAQAAYEYEAQLQANFPYSEELQTVLT

General information: TITO was launched using: RESULT: Template: 2VQ2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 833 8389 10.07 45.10 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 10.07 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.283

(partial model without unconserved sides chains): PDB file : Tito_2VQ2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VQ2-query.scw PDB file : Tito_Scwrl_2VQ2.pdb: