Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------MRKINFFDTTLRDGEQSPGVNLNT-QEKLAIA-KQLERL-GADIIEAGFPASSRGDFLAVQEIARTI---KNCSVTGLARCVKGDIDAAWEALKDGAQPRIHVFIATSDIHLKHKLKMTREQVIERAVGMVKYAKERFP-------IVQWSAEDACRTELPFLAEIVEKVIDAGA------SVINLPDTVGY-LAPAEYGNIFKY-MKENVPNIHKAKLSAHCHDDLGMAVANSLAAIENGADQIECAVNGIGERAGNAALEEIAVALHTRKDFYQVETGITLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQDGFLKEKST-YEIISPELVGVTA-DA-----LVLGKHSGRHAFKDRLTALGFQ-FDSEEINKFFTMFKELTEKKK--EITDED-LVSLILEEKVTDRKIGYEFLSLQVHYGTSQVPTATLSLKNQENAQLIQEAATGAGSVEAIYNTLERCIDKDVELLDYRIQS-NRKGEDAFAQVYVRVLVNG-KESAGRGIAQDVLEASAKAYLNAVNRQLVFQSNMSGLKNHTAVGS 1SR9 Chain:A ((18-643)) TIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRNRTWPDRVIDRAPLWCAVDLRDGNQALI---DPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSGAPRAIVHFYNSTSILQRRVVFRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIAPTPERPIIFNLPATV-EMTTPNVYADSIEWMSR-NLANRESVILSLHPHNDRGTAVAAAELGFAAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRG----VDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCDVDDMLWQVPYLPIDPRDVGRTYEAVIKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIEVSPKEMWDAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKI--NGVETEISGSGNGPLAAFVHALAD-VGFDVAVLDYYEHAMS-AGDDAQAAAYVEASVTISKTVWGVGIAPSITTASLRAVVSAVNRAA-----------------

General information: TITO was launched using: RESULT: Template: 1SR9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2339 54808 23.43 112.31 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 23.43 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_1SR9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SR9-query.scw PDB file : Tito_Scwrl_1SR9.pdb: