Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKRKIGLAMSLVIAAGTILGACGNSEKSSGSGEGKNKFSVAMVTDVGGVDDKSFNQSAWEGIQAFGKENGLKKGKNG-YDYLQSKSDADYTTNLNKLARENFDLIYGVGYLMED---SISEIADQRKNTNFAIIDAVVDK------DNVASITFKEQEGSFLVGVAAALSSKS-----GKIGFVGGME--SELIKKFEVGFRAGVQAVNPKA-VVEVKYAGGFDKADVGKATAESMYKS----GVDVIYHSAGATGTGVFTEAKNLKKEDPKRDVWVIGVDKDQYAEGQVEGTDDN--VTLTSMVKKVDTVVEDVTKKASDGKFPGGETLTYGLDQDGVGISPSKQNLSDDVIKAVDKWKKKIIDGLEIPATEKELKTFKAE 4KZK Chain:A ((37-304)) --------------------------------------VKIGFVV-KQP--DDPWFQDEWRFAEQAAKDK------HFTLVKIAAPSGEKVSTALDSLAAQKAQGVIICAPDVKLGPGIAAKAKR--YGMKLMSVDDQLVDGRGAPLADVPHMGISAYRIGRQVGDAIAAEAKRRGWNPAEVGVLRLAYDQLPTARERTTGAVDALKAAGFAAANVVDAPEMTA-DTEGAFNAANIAFTKHRNFRHWVAFGSNDDTTVGAVRAGEGRGIGT--DDMIAVGINGSQVALNEF--AKPKPTGFFGSILLNPRLHGYDTSVNMYD-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4KZK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1275 21850 17.14 89.55 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 17.14 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_4KZK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KZK-query.scw PDB file : Tito_Scwrl_4KZK.pdb: