Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLVLIGGGYGNMRVLHRLLPNQLPDDVSITLIDRNPYHCLK-TEYYALAAGTISDHHIR-VSFPEH---PRLDVQY-GDITSIDIVQKQVLFQDREPISYDDAIIGLGCEDKYHNVPGAPE-FTYSIQTIDQSRETYQKLNNLSANATVAIVGAGLSGVELASELRESRDDLNIILFDRGNLILSS-FPERLSKYVQKWFEEHGVRIINRANITKVEEG-----VVYNHDDPISADAIVWTAGIQPN-KVVRDLDVEKDAQGRIVLTPHHNLPGDEHLYVVGDCASLPHAPSAQ--LAEAQAEQIVQILQKRWNGEALPESMPQFKLKGVLGSLGKKAGFGLVADRPLIGRVPRMLKSGLLWMYKHHNG 2V3A Chain:A ((4-306)) RAPLVIIGTGLAGYNLAREWRKLD--GETPLLMITADDGRSYSKPMLSTGFSKNKDADGLAMAEPGAMAEQLNARILTHTRVTGIDPGHQRIWIG-EEEVRYRDLVLAWGAEPIRVPVEGDAQDALYPINDLEDYARFRQA---AAGKRRVLLLGAGLIGCEFANDLSSGG--YQLDVVAPCEQVMPGLLHPAAAKAVQAGLEGLGVRFHLGPVLASLKKAGEGLEAHLSDGEVIPCDLVVSAVGLRPRTELAFAAGLAVN--RGIVVDRSLR-TSHANIYALGDCAEVDGLNLLYVMPLMACARALAQTLAGN---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2V3A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1555 -39346 -25.30 -137.09 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -25.30 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_2V3A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V3A-query.scw PDB file : Tito_Scwrl_2V3A.pdb: