Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIAVINGGTRSG-GNTDVLAEKAVQGF-------DAEHIYLQKYPIQPIEDL-----RHAQG--GFRPVQDD---YDSIIERILQCHILIFATPIYWFGMSGTLKLFIDRWSQTLRDPRFPD----FKQQMSVKQAYVIAVGGDNPKI------KGLPLIQQFEHIFHFMGMSFKGYVLGEGNRPGDILRDHQALSAASRLLKRSDAI 4N65 Chain:A ((2-210)) SRILAVHASPRGERSQSRRLAEVFLAAYREAHPQARVARREVGRVPLPAVTEAFVAAAFHPQPEQRSLAMQADLALSDQLVGELFDSDLLVISTPMYNFSVPSGLKAWIDQIVRLGVTFDFVLDNAQYRPLLRGKRALIVTSRGGHGFGPGGENQAMNHADPWLRTALGFIGIDEVTVVAAEGEESG---SFEDSCDEAEQRLLALAR-

General information: TITO was launched using: RESULT: Template: 4N65.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 807 -2448 -3.03 -13.83 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -3.03 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_4N65.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N65-query.scw PDB file : Tito_Scwrl_4N65.pdb: