Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGIKQYSQEELKEMALVEIAHELFEE-HKKPVPFQELLNEIASLLGVKKE-----ELGDRIAQFYTDLNIDGRFLALSD------QTWGLRSWYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK 2MBF Chain:A ((346-427)) ------------AESYSDLIAKALKSTFDGRMRLNEIYNWFASNVPYFGNRTSQEQSAGWKNSIRHNLSLHSRFMRIQNEGAGKSSWWVINPDA-------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2MBF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 172 -7554 -43.92 -107.91 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -43.92 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.562

(partial model without unconserved sides chains): PDB file : Tito_2MBF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MBF-query.scw PDB file : Tito_Scwrl_2MBF.pdb: