Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKRTAFVMGASQGIGKAIALKLADQHFSLVINSRNLDNIESVKEDILAKHPEASVIVLAGDMSDQHTRAGIFQKIESQCGRLDVLINNIPGGAP--DTFDNCNIEDMTATFTQKTVAYIDAIKRASSLMKQNEFGRIINIVGNLWKEPGANMFTNSMMNAALINASKNISIQLAPHNITVNCLNPGFIATDRYHQFVENVMKKNSISKQKAEEQIASGIPMKRVGSAEETAALAAFLASEEASYITGQQISADGGSMKSI 3TZQ Chain:B ((9-252)) LENKVAIITGACGGIGLETSRVLARAGARVVLADLPETDLAGAAASVG-----RGAVHHVVDLTNEVSVRALIDFTIDTFGRLDIVDNNAAHSDPADMLVTQMTVDVWDDTFTVNARGTMLMCKYAIPRLISAGGGAIVNISSATAHAAYDMSTAYACTKAAIETLTRYVATQYGRHGVRCNAIAPGLVRTPRLE---PQ----------PIVDIFATHHLAGRIGEPHEIAELVCFLASDRAAFITGQVIAADSGLLA--

General information: TITO was launched using: RESULT: Template: 3TZQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1356 -72274 -53.30 -302.40 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -53.30 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.562

(partial model without unconserved sides chains): PDB file : Tito_3TZQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TZQ-query.scw PDB file : Tito_Scwrl_3TZQ.pdb: