Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREELKGIFASVFGEKEGLRFFFAPGRVNLIGEHTDYNGGH-VFPCALTMGTYAAVAERNDGLVRMYSDNFRNAGIKECSLDDIRYQKEDDWANYPKGVIYEFQQRG---YAVPHGFDIVFSGNIPN---GAGLSSSASIELLMGVVLQSYFHPEVDALELVKMAQHAENHFIGVNCGIMDQFAIGMGKKHHAMLLNCDTLD-Y------EYSK---------------LNVSGLALVIANTNKKRTLADSSYNTRRQECNDALLDLKKGLDIAALGDIKPSDFDAHSSLIQNETNRRRAKHAVYENHRAIKTAHMFKENNIDEIGQLMKESHLSLKDDYE-VTCPELDELVFAAWDHEGVIGSRMTGAGFGGCTISIVKDE-FVDDFIQKVGDRYQEKTGLRADFYVADIGEGARELKGE 3GON Chain:A ((2-317)) -------------------IAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSYRIY--SDMFDFA----VD------LRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLAIYGKMEREGKKFGLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLK-RGDNGSMGDLACIAAEDL---VLYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPT-LECDFLVGWTKEVAVSSH------------MVQQIKQNIN---------QN---F---------LTSSKE------TVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQLKEASQD-LQA-VAKSSGAGGGDCGIALSFDAQSTKTLKNRWA----------------------------

General information: TITO was launched using: RESULT: Template: 3GON.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1420 -25254 -17.78 -88.61 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -17.78 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_3GON.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GON-query.scw PDB file : Tito_Scwrl_3GON.pdb: