Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKKWQWLV----YGWKLIIL---TLLTAAVFSYAMFQGGFVSWFLFYAF--LPFVLYAGLLALYPLRSFQASRQMDKTQLHAGDRLGVTVTLRRKLPFPLMYMVIEDCLPEGIESLNRDGAAAKRLVF--PWFKRSMTMSYELARVPRGEHHFHSVRVRTGDVLGLIEKTAFFELDDTLFVYPFYQRFSYQVNERHQEDGVSGSSPIHQHHSSVAASVRNY--QPGDRFAALDWKTSARRSQLMTKEFE-PSRSKNLFLLMDRFSSDAFEEVVSVTASILHSV--------------LKNGAGAGLASIGKEKNIFPIQE-GDQHFKHMLRHLAIAHCD------AADPISRYAREE---LGKPSVRQADQV----VVTGQLTEDMLHLAEIGGGRVTVILAKEKDAELSQAEN------VMIERMMKRQIRVRIM---RGGRVSRVV--------- 4C0H Chain:A ((19-445)) GDNEWHKLVIPKGSDWQIDLKAEGKLIVKVNSGIVEIFGTELAVDDEYTFQNWKFPIYA----------------VEETELLWKCPDLTTNTITVKPNHTMKYIYNLHFMLEKIRMSNFEGP---RVVIVGGSQTRKTSLSRTLCSYALKFNAYQPLYIN------LDPQQPIFTVPGCISATPISDILDAQLPTWGQSL-TSGATLLHNKQP----MVKNFGLERINENKDLYLECISQLGQVVGQRLHLDPQVRRSGCIVDTPSISQLDENLAELHHIIEKLNVNIMLVLCSETDPLWEKVKKTFGPELGNNNIFFIPKLVDDVYKRSLQRTSIREYFYGSLDTALSPYAIGVDYEDLTIWKPSNVFDNEVGRVELFPVTITPSNLQ------HAIIAITFAERRADQATVIKSPILGFALITEVNEKRRKLRVLLPVPGRLPSKAMILTSYRYLE

General information: TITO was launched using: RESULT: Template: 4C0H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1673 -173010 -103.41 -477.93 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -103.41 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.246

(partial model without unconserved sides chains): PDB file : Tito_4C0H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C0H-query.scw PDB file : Tito_Scwrl_4C0H.pdb: