Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKHISMLFVFLMAVMVLSACNSSESSSNSEVSSSKTRTVKHAMGTSDNIPANPKRIVVLTNEGTEALLALGI--KPVGAVKSWKGDPWYDYLKDDMKGVKNVGLETEPNVEAIAELKPDLIIGNKVRQEKIYDQLNAIAPTVFAES----LAGNWKDNLTLYANAVNKA--DKGKEVIADFDKRVSDLKNKLGDQTNKTVSVVRFLSGESRIYYTDSFPGIILDQLGFKRPEKQVELFKKQKDQFTFSTDSKESIPDMDADVLFYFTYKADNAKENEKWANQWTSSSLWKNLKAVKSGNAHEVDDVVWTT-AGGIKAANYLLDDIETYFLKTK 4JCC Chain:A ((4-285)) ----------------------------------PTEVTIKSS-LDEVKLSKVPEKIVTFDLGAADTIRALGFAKNIVGMPTKT----VPTYLKDLVGTVKNVGSMKEPDLEAIAALEPDLIIASPRT-QKFVDKFKEIAPTVLFQASKDDYWTSTKANIESLASAFGETGTQKAKEELAKLDESIQEVATKNESS-DKKALAILLNEGKMAAFGAKSRFSFLYQTLKFKPTDTKFE------DSRHGQEVSFESVKEINPDILFVINRTLAIG-GDNSSNDGVLENALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKAL----

General information: TITO was launched using: RESULT: Template: 4JCC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1483 -134046 -90.39 -491.01 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -90.39 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_4JCC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JCC-query.scw PDB file : Tito_Scwrl_4JCC.pdb: