Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVNGIHHVSALTADAQKNLDFYKKVLGLKLVKKSVNQDEPTMYHLFYGDEVANPGTELTFFEIPRIAPFHAGTNSISSIGLRVPGTEALHYWKERFEEQQVTHSG-I----SKRAGRDILAFQDHEGQRLVLTADEEGKGYGLPVKQS-G-IPEEFSFRGLGPVELTVPYAEPTLHVLTNILGFTEISREPVEGQG----TAVILESGEGGAATEIHLIERNDLPRERQGKGSVHHVAFRVRDEEELAGWHRIISREGFSNSGIVE--RYYFKALYFREPNGILFELSTDGPGFMVDENLDELGQTIALPPYLEHRRAEIEAKLKPIQ 1LGT Chain:A ((1-277)) -SIRSLGYMGFAVSDVAAWRSFLTQKLGLMEAGTTDNG-------DLFRIDS---RAWRIAVQQ-------GEVDDLAFAGYEVADAAGLAQMADKLKQAGIAVTTGDASLARRRGVTGLITFADPFGLPLEIYYGASEVF-EKPFLPGAAVSGFLTGEQGLGHFVRCVPDSDKALAFYTDVLGFQLSDVIDMKMGPDVTVPAYFLHCN--ERHHTLAIAAF-------PLPKRIHHFMLEVASLDDVGFAFDRVDADGLITSTLGRHTNDHMVSFYASTPSGVEVEYGWSARTVDRSWVVVRHD------------------------

General information: TITO was launched using: RESULT: Template: 1LGT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1480 -123138 -83.20 -466.43 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -83.20 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_1LGT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LGT-query.scw PDB file : Tito_Scwrl_1LGT.pdb: