Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQSLTLIPTLREVPADAEAKSHQLLLRAGFIRQ-NTSGVYSYMPLAYKVIQNIQQIVREEMEKIDAVEMLMPALQQAETWQESGRWYTYGPELMRLKDRH----GREFALGATHEEVITSLVRDEVKSYKRLPLTLYQIQSKFRDEKRPRFGLLRGREFIMKDAYSFHASAESLDETYQKMYEAYSNIFARCG-INVRPVIADSGAMGGKDTHEFMALSAIGEDTIAYSDESQYAANIEMAEVLHQEVPSDEEPKALEKVHTPNVKTIEELTAFLQVSAEACIKSVLFKADDRFVLVLVRGDHEVNDIKVKNLLHAEVVELATHEEVIQQLGTEPGFVGPVGIHQDVEVYADQAVKAMVNAVAGANEGDHHYKNVNVNRDAQIKEFADLRFIKEGDPSPDGKGTIRFAEGIEVGQVFKLGTRYSEAMNATYLDENGRAQPMLMGCYGIGVSRTLSAIAEQHHDEKGLIWPKSVAPYDLHILALNMKND--GQRELAEKLYADLKAEGYEVLYDDRA-ERAGVKFADSDLIGLPIRITVGKRA-DEGIVEVKIRQTGESTEISVDELSAFISKQ 1HC7 Chain:A ((17-381)) --------------------WYLEVIQKAELADYGPVRGTIVVRPYGYAIWENIQQVLDRMFKETGHQNAYFPLFIPMSFL--------FSPELAVVTHAGGEELEEPLAVRPTSETVIGYMWSKWIRSWRDLPQLLNQWGNVVRWEMRT-RPFLRTSEFLWQEGHTAHATREEAEEEVRRMLSIYARLAREYAAIPVIE----------------------GLKT-----------EKEKFA--------------------------------------------------------------------------------------------GAV---------------------------------YTTT------------IEA--------------LMKDGKALQAGTSHYLGENFARAFDIKFQDRDLQVKYVHTTSWGLS-WRFIGAIIMTHGDDRGLVLPPRLAPIQVVIVPIYKDESRERVLEAAQGLRQALLAQGLRVHLDDRDQHTPGYKFHEWELKGVPFRVELGPKDLEGGQAVLASRLGG-KETLPLAALPEAL---

General information: TITO was launched using: RESULT: Template: 1HC7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1785 -172706 -96.75 -499.15 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -96.75 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.592

(partial model without unconserved sides chains): PDB file : Tito_1HC7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HC7-query.scw PDB file : Tito_Scwrl_1HC7.pdb: