Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTKTAFMAILFSLITVLSACGAGSQTTGAGQKKVQTITVGTGTQFPNICFIDEKGDLTGYDVELIKELDKRLPHYKFTFKTMEFSNLLVSLGQHKVDIVAHQMEKSKEREKKFLFNKVAYNHFPLKITVLQNNDTIRGIEDLKGKRVITSATSNGALVLKKWNEDNGRPFEIAYEGQGANETANQLKSGRADATISTPFAVDFQNKTST-IKEKTVGNVLSNAKVYFMFNKNEQTLSDDIDKALQEIIDDGTLKRLSLKWLGDDYSKEQY 3H7M Chain:A ((7-232)) -------------------------------RDRHRTIVVGGDRDYPPYEFIDQNGKPAGYNVELTRAIAEVM-GMTVEFRLGAWSEMFSALKSGRVDVLQ-GISWSEKRARQIDFTP-PHTIVYHAIFARRDSPPAAGLEDLRGRKVALHRDGIMHEYLAER----GYGKDLVLTP-TPADALRLLAAGGCDYAVVAMVPGMYIIRENRLTNLVPVARSIAAQRYGYAVRQGDAELLARFSEGLAILRKTGQYEAIRAKWLGVL------

General information: TITO was launched using: RESULT: Template: 3H7M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1225 -90297 -73.71 -401.32 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -73.71 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.362

(partial model without unconserved sides chains): PDB file : Tito_3H7M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H7M-query.scw PDB file : Tito_Scwrl_3H7M.pdb: