Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIFRKKKARRGLAQISVLHNKSVVAEQYRTIRTNIEFSSVQTNLRSILVTSSVPGEGKSFSAANLAAVFAQQQEKKVLLVDADLRKPTINQTFQVDN--VTGLTNVLVGNASLSET-VQKTPIDNLYVLTSGPTPPNPAELLSSKAMGDLISEIYEQ-FSLVIFDSPPLLAVADAQILANQTDGSVLVVLSGKTKTDTVLKAKDALEQS-----------NAKLLGALLNKKKMKKSEHYSY-------------------------------------------------------------- 3Q9L Chain:A ((2-258)) -------------------------------------------ARIIVVTSGKGGVGKTTSSAAIATGLAQK-GKKTVVIDFAIGLRNLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDA--LTREGVAKVLDDLKAMDFEFIVCDSPAGIE-TGALMALYFADEAIITTNPEVSSVRDSDRILGILASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVLRASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEE

General information: TITO was launched using: RESULT: Template: 3Q9L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 987 38 0.04 0.21 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 0.04 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_3Q9L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q9L-query.scw PDB file : Tito_Scwrl_3Q9L.pdb: