Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKNEILWGIAPIGWRNDDMPEIGAGNTLQHLLSDIVVARFQGTEVGGFF-----P---EPAILNKELKLRNLRIAGKWFSSFILRD-G-LGEAAKTFTLHCEYLQQVNADVAVVSEQTYSVQSLEKNVFTEKPHFTDDEWERLCEGLNHLGEIAAQHGLKLVYHHHLGTGVQTAEEVDRLMAGTDPAHVHLLYDTGHAYISDGDYMGMLEKHIGRIKHVHFKDARLNVMEQCRLEGQSFRQSFLKGMFTVPGDGCIDFREVYQLLLKHSYSGW-IVIEAEQDPDVA-NPLEYALIARNYIDQQLLDLA 2G0W Chain:A ((35-289)) -------------------------EVSFPKRVKVAAENGFDGIGLRAENYVDALAAGLTDEDMLRILDEHNMKVTEVEYITQWGTAEDRTAEQQKKEQTTFHMARLFGVKHINCGLL---------------E---KIPEEQIIVALGELCDRAEELI--IGLEFMPYSGVADLQAAWRVAEACGRDNAQLICDTWHWARANQTAESIKNVPADRIVSIQLCDVHETPYKELR--------EESLHDRLAPGEGYGDTVGFAKILKEHGVNPRVMGVEVISDSMVATGLEYAALKVYNATKKVLDEA-

General information: TITO was launched using: RESULT: Template: 2G0W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1246 17433 13.99 71.74 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 13.99 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.418

(partial model without unconserved sides chains): PDB file : Tito_2G0W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2G0W-query.scw PDB file : Tito_Scwrl_2G0W.pdb: