Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTREDIFKHVKEKYGTSPDYPWEKYPNYASLRHTSNKKWYGLIMNVLPEKLGLDGHGEIDILNLKCPPEISDRLRNG-ENILPGYHMDKEHWISIVLERTDPEGEIYNLIEQSFHLTK 2FKI Chain:A ((1-104)) -MTISELLQYCMAKPGAEQSVHNDW--KATQIKV--EDVLFAMVKEVE-------NR---PAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQAV

General information: TITO was launched using: RESULT: Template: 2FKI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 398 -24942 -62.67 -242.15 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -62.67 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_2FKI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FKI-query.scw PDB file : Tito_Scwrl_2FKI.pdb: