Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGISNFKTPSKLSEKKKSVLCSTPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGSLCLAMEYGGEKSLNDLIEERYKASQDPFPAAIILKVALNMARGLKYLHQEKKLLHGDIKSSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINME-ELDESYQKVIELFSVCTNEDPKDRPSAAHIVEALETDV 4FIE Chain:A ((155-400)) ---------------------------------NFIKIGEGSTGIVCIATVRSSG---KLVAVKKMDLRKQQ-----RRELLFNEVVIMRDYQHENVVEMYNSYLV-GDELWVVMEFLEGGALTDIVTH------TRMNEEQIAAVCLAVLQALSVLHA-QGVIHRDIKSDSILLTHD-GRVKLSDFGFCAQVSKEVPRR---KSLVGTPYWMAPELISRL-PYGPEVDIWSLGIMVIEMVDGEPPYFNEPP--------------LKAMKMIRDNLPPRLKNLHKVSPSLK---GFLDRLLVRDPAQRATAAELLKHPFLAK

General information: TITO was launched using: RESULT: Template: 4FIE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -74197 for 1915 contacts (-38.7/contact) + 2D Compatibility (PS) -26948 + (NN) -17871 + (LL) 2764 1D Compatibility (HY) -18000 + (ID) 2650 Total energy: -136902.0 ( -71.49 by residue) QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_4FIE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FIE-query.scw PDB file : Tito_Scwrl_4FIE.pdb: