Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLVEDDHSISEMVDHYLTKEGFGIVHAFDGEEGIRLFQQGSYDLVLLDIMLPKLNGMDFLKIIREKSN-IPVLMISAKDGDVDKALGLGFGADDYIAKPFSMIELTARVKAAIRR--ATQYSAEEPAVNKVIRIHQLAIDIDNVSVLKNGEPLQLTSTEWQLLCLFASNPKKVFTKEQIYRSVWNEEYFDDQNIINVHMRRLREKIEDDPSSPQYIKTLWGIGYKLGEF 1YS6 Chain:A ((23-230)) ------------LERGLRLSGFEVATAVDGAEALRSATENRPDAIVLDINMPVLDGVSVVTALRAMDNDVPVCVLSAR--VDDRVAGLEAGADDYLVKPFVLAELVARVKALLRRRGSTATSSSE-----TITVGPLEVDIPGRRARVNGVDVDLTKREFDLLAVLAEHKTAVLSRAQLLELVWGYDFAADTNVVDVFIGYLRRKLEAG-GGPRLLHTVRGVGFVL---

General information: TITO was launched using: RESULT: Template: 1YS6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 980 -142645 -145.56 -702.68 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -145.56 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.403

(partial model without unconserved sides chains): PDB file : Tito_1YS6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YS6-query.scw PDB file : Tito_Scwrl_1YS6.pdb: