Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSGVIPSSAVGQKINEWYRYIRTFSVPDAEVLKAEIQQELKHMQHDSNLLLYYSLMEFRHQLMLDYLEPLEKLNIEDQPSLSELSRNIDSNQADLKGLLDYYVNFFRGMYEFDKREFISAITYYKQAEKKLSFVADHIERAEFYFKIAEAYYYMKQTYFSLINIKNAYEIYVEQETYNVRIIQCHFVFGVNLMDERNFEQAARHFKLALNMAQAEQK-AQLVGRAYYNLGLCYYNQDLLDPAIDYFEKAVSTFESSRIVNSLPQAYFLITLIYYKQGKHDKASEYHKRGYEYAKETDDADYAV-KFEFLQSL-YL--DQPNEEGIERCFQYLKNKNMYADIEDLALEVAKYYYEQKWFKLSASYFLQVEEARKQIQRSEGLYEIEI 4GPK Chain:A ((8-359)) -----DVEEDVKGKLDEWLNALVHLDKQQVERIYEELQGEMKH-VLDFEIINYYKLLYTRYLIMKRDISALEEEL-D------KL-KKV-YK--KYSPFQKLLYMYGRGLLCCLQYRWKDGLDYLLKTEVMAKEQG--YHETGLYYNIALAYTHLDIHHLAIHFVNMALEGFRSE-YKFRNIINCQILIAVSYTEKGQYEEALKMYESILREATSFADKDVLLAITLSNMGSIYYKKGKYQQAKKYYLDSLQLQKQIDL--NYLDTIYEMALVCIKLEELEEARTLIDKGIDAAKQEERFNAKLYLLLMLRYKYFEEAKDYKAFLENEAIPLYK----------VYVELAEHFSSLSRFEESNRYYRLVIDLMN-------------

General information: TITO was launched using: RESULT: Template: 4GPK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1317 -165375 -125.57 -490.73 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -125.57 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_4GPK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GPK-query.scw PDB file : Tito_Scwrl_4GPK.pdb: