Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKTMGIHHITAIVGHPQENTDFYAGVLGLRLVKQTVNFDDPGTYHLYFGNEGGKPGTIITFFPWAGARQGVIGDGQVGVTSYVVPKGAMAFWEKRLEKFNVPYTKI-----ERFGEQYVEFDDPHGLHLEIVEREEGEANTWTFGEVTPD-----------VAIKGFGGATLLSEQPDKTADLLENIMGLERVGKEGDFVRYRSAGDIGNVIDLKLTP------IGRGQMGAGTVHHIAWRANDDEDQLDWQRYIASHGYGVTPVRDRNYFN---AIYFREH-GEILFEIATDPPGFAHDETQETMGEKLMLPVQYEPHRTQIEQGLLPFEVRELD 1MPY Chain:A ((1-307)) MNKGVMRPGHVQLRVLDMSKALEHYVELLGLIEMDRD----DQGRVYLKAWTEVDKFSLVLREAD---------EPGMDFMGFKVVDEDALRQLERDLMAYGCAVEQLPAGEL-NSCGRRVRFQAPSGHHFELYADKEYTGKWGLNDVNPEAWPRDLKGMAAV----RFDHALMYGDELPATYDLFTKVLGF----YLAEQVLDE---NGTRVAQFLSLSTKAHDVAFIHHPEKGRLHHVSFHLETWEDLLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPS-GNRNEVFCGGDYNYPDHKPVTWTTDQLGKAIFYHDRILNERFMTVLT-----

General information: TITO was launched using: RESULT: Template: 1MPY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1484 -105811 -71.30 -376.55 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -71.30 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.330

(partial model without unconserved sides chains): PDB file : Tito_1MPY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MPY-query.scw PDB file : Tito_Scwrl_1MPY.pdb: