Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVMLATALFLGLTPAGANAADLGHQTLGSNDGWGAYSTGTTGGSKASSSNVYTVSNRNQLVSALGKET-------------NTTPKIIYIKGTIDMNVDDNLKPLGLNDYKDP------------EYDLDKYLKAYDPSTWGK-KEPSGTQEEARARSQKNQKARVMVDIPANTTIVGSGTNAKVVGGNFQIK-----SDNVIIRNIEFQDAYDYFPQWDPTDGSSGNWNSQYDNITINGGTHIWIDHCTFNDGSRPDSTSP------------KYYGRKYQHHDGQTDASNGANYITMSYNYYHDHDKSSIFGSSDSKTSDDG---KLKITLHHNRYKNIVQRAPRVRFGQVHVYNNYYEGSTSSSS-YPFSYAWGIGKSSKIYAQNNVIDVPGL-SAA---KT------ISV-----FS-----GGTALYDSGTLLNGTQINASA----ANGLSSSVGWTP--SLHGSIDASANVKSNVINQAGAGKLN 4HWV Chain:A ((10-484)) ------------------NSAAPAGSIDSLGATGWASHGTPTTGGQGAAAERTYTVANRNELIQALYGNTAVIAPDGSVQGTPDKAPKVIRIRGTIDLNVDGQLRPYTPDRYVAGSCASSVHGYASQASLWSDYLAAYRPGAWGNARTVSGKPEDARACAAELQRRVVTISVPDNTSLLGIGTDAKILHGNLMLGTPDAPVANIVIRNITFEDAFDDFPQWDPTDSSDGRWNSEYDLISVAHASHVWIDHNTFSDGDRHDHAFPSVWHETVHGTDYSGGDFKVQHHDGLVDVTRHGNYVTLSNNHFHDHDKAFLIGGTDVPGADSGNPRMLKVTFHGNHFQNLRQRQARVRYGMVHLYNNYYENTRDASADYPWLAGMTLGQSGKVHAENNVVSLAGPDRPARPADVANARISAARTQDCAALFSASECASTFYDSGTVLNGGPADLTAAVRWSSALAAAPAWKPSDFYDYTLEDTADLAARITARAGAGKLE

General information: TITO was launched using: RESULT: Template: 4HWV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2519 -81316 -32.28 -202.28 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -32.28 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.690

(partial model without unconserved sides chains): PDB file : Tito_4HWV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HWV-query.scw PDB file : Tito_Scwrl_4HWV.pdb: