Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------MVEKCYSCLICGYKGL----IQNPLYKGEYQKT-FDICPCCGFEFGYSEDHDVRLGFIVT--PDHLI--EAAFQLYRKQWLESGMVIAHPEDIPEELKNGNCLKFEVLLKQLKKLNLDIENFEISGF--------------- 4D0Y Chain:A ((1-184)) AEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLIKAMQEAGFP-ISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSE-HQTGLAFDVIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETG------YMAEEWHLRYVGKEAKEIAESGLSLEEYYGFEGGDYVD

General information: TITO was launched using: RESULT: Template: 4D0Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 376 3673 9.77 33.39 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 9.77 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.313

(partial model without unconserved sides chains): PDB file : Tito_4D0Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D0Y-query.scw PDB file : Tito_Scwrl_4D0Y.pdb: