Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------------------------------------------------------------MGRHKAT-IEGLVMKERYYSHRAPG-----TERWITQPVCKVTRTEPIFEGYI--DIE-PIEIGGKVYIPGLNEYVI--VTDRQRNIHNEWT---YQTDRVIKTIIDEKSLKECEEHNNKKAKNNDTQNQRQIKTSWWQRLTKKD------------------------------------------------------------------ 2BBD Chain:A ((1-323)) GEIYTETLQQTYAWTAGTNIPIKIPRNNFIRKIRVQLIGSISNSGTAAVTLPSAPFPYNLVQTFNLSYEGSKTLYSVSGTGLGILMYYTTKGQNPAYPAPGTSVPASGSVNLNVMWEFDLARFPATMVQNIILS--ILTGQAPSGVSINASFYITITYERVTAQEILSEGGLGADGEMPLA----TVLPKVIEIPTFNVPASSAPIHVAYLQPGQIYKRQLVYVINSTSGINNTDPTEYELKIVRGVPTDKIKVS-WAALQAENQAEYQVAPYSGASAIIDFRKYFNGDLDLTHAPSDSIEYDLALQNQDNVYSLYVSYVLPYYDQLAAL

General information: TITO was launched using: RESULT: Template: 2BBD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 436 26725 61.29 215.52 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 61.29 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.088

(partial model without unconserved sides chains): PDB file : Tito_2BBD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BBD-query.scw PDB file : Tito_Scwrl_2BBD.pdb: