Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVSIRRSFEAYVDDMNIITVLIPAEQKEIMTPPFRLETEITDFPLAVREEYSLEAKYKYVCVSDHPVTFGKIHCVRASSGHKTDLQIGAVIRTAAFDDEFYYDGELGAVYTAD--HTVFKVWAPAATSAAVKLSHPNK---SGRTFQMTRLEKGVYAVTVTG-------DLHGYEYLFCICNNS---------------------------EWMETVDQYAKAVTVNGEK--------------------------GVVLRPDQMKWTA-PLKPFSHPVDAVIYETHLRDFSIHENSGM-INKGKYLALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEE----KPLDAYNWGYNPLHFFAPEGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKRENSPFEKT------------VP-GYFFRHDECGMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNVDGFRFDLLGILDIDTV-------------------------------LYMKEKATKAKPGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNTFHLK-ATGFALGNGESAQAVMHGIAGSSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQE----------------------------NDSRKRSRQRLAAAIILLAQGVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDRRETFKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECL-------------TLKEHLIAYRLYDLDEVDEWKDIIVIHHASPDSVEWRLPN---DIPYRLLCDPSGFQEDP-----------TE-IKKTVAVNGIGTVILYLASDLKSFA 1BF2 Chain:A ((6-749)) --------------------------------------------------------------------------------------------------------SLGASYDAQQANITFRVYSSQATRIVLYLYSAGYGVQESATYTLSPAGSGVWAVTVPVSSIKAAGITGAVYYGYRAWGPNWPYASNWGKGSQAGFVSDVDANGDRFNPNKLLLDPYAQEVSQDPLNPSNQNGNVFASGASYRTTDSGIYAPKGVVLVPS--TQSTGTKPT-RAQKDDVIYEVHVRGFTEQDTSIPAQYRGTYYGAG-----------LKASYLASLGVTAVEFLPVQETQNDANDVVPNSDANQNYWGYMTENYFSPDRRYAYNKAAG-GPTAEFQAMVQAFHNAGIKVYMDVVYNHTAEG-GTWTSSDPTTATIYSWRGLDNATYYELTSGNQYFYDNTGIGANFNTYNTVAQNLIVDSLAYWANTMGVDGFRFDLASVLGNSCLNGAYTASAPNCPNGGYNFDAADSNVAINRILREFTVRPAAGGSGLDLFAEPWAIGGNSYQ-LG-G--F---PQGWSEWNGLFRDSLRQAQNELGSMTIYVTQ-------DANDFSGSSNLF--QSSGRSPWNSINFIDVHDGMTLKDVYSCNGANNSQAWPYGPSDGGTSTNYSWDQGMSAGTGAAVDQRRAARTGMAFEMLSAGTPLMQGGDEYLRTLQCNNNAYNLDSSANWLTYSWTTDQSNFYTFAQRLIAFRKAHPALRPSSWYSGS-QLTWYQPSGAVADSNYWNNTSNYAIAYAINGPSL-GDSNSIYVAYNGWSSSVTFTLPAPPSGTQWYRVTDTCDWNDGASTFVAPGSETLIGGAGTTYGQCGQSLLLLIS--------

General information: TITO was launched using: RESULT: Template: 1BF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3473 10932 3.15 19.11 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 3.15 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_1BF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BF2-query.scw PDB file : Tito_Scwrl_1BF2.pdb: