Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELYIITGASKGLGQAIALQALEKGHEVHALSRTKTD------VSHK---KLTQHQIDLINLEEAEQQFETLLSSIDSDRYSGITLINNAGMVTPIKRAGEASLDELQRHYQLNLTAPVLLSQLFTKRFASYSGKKTVVNITSGAAKNPYKGWSAYCSSKAGLDMFTRTFGFEQEDEELPVNMISFSPGVMDTEMQAVIRSSSKKDFHHIERFRKLNETGSLRSPDFIAGTLLSLLEKG--TENGRIYDIKEFL 4G81 Chain:D ((9-248)) GKTALVTGSARGLGFAYAEGLAAAGARVILNDIRATLLAESVDTLTRKGYDAHGVAFDVTDELAIEAAFSKLDAE----GIHVDILINNAGIQYR-KPMVELELENWQKVIDTNLTSAFLVSRSAAKRMIARNSGGKIINIGSLTSQAARPTVAPYTAAKGGIKMLTCSMAAEWA--QFNIQTNAIGPGYILTDMNTALIED--K--QFDSWVKSSTPSQRWGRPEELIGTAIFLSSKASDYINGQIIYVD---

General information: TITO was launched using: RESULT: Template: 4G81.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1172 10631 9.07 46.42 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.71 3D Compatibility (PKB) : 9.07 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_4G81.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G81-query.scw PDB file : Tito_Scwrl_4G81.pdb: