Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALNKPKIVILGAGYGGLMTVTRLTKYVGPNDADITLVNKHNYHYETT-WM-HEASAGTLHHDRCRYQIKDVINQSRVNFVQDTVKAIKIDEKKVVLANG-ELQYDYLVIGLGAVPETFGIKGLK-EYAFPIANINTSRLLREHIELQFATYNTEAEKRPDRLTIVVGGAGFTGIEFLGELAARVPELCKEYDVDRSLVRIICVEAAPTVLPG-FDPELVDYAVHYLEENGVEFKIGTAVQECTPE-----GVRVGKKDEEPEQIKSQTVVWAAGVRGHPIVEE-AGFENMRGRVKVNPDLRAPGHDNVFILGDSSLFMNE-DTERPYPPTAQIAMQQGITVAKNLGRLIKGGELEEFKPDIKGTVASLGEHNAVGVVYGRKLKGTPASFMKKVIDNRSLFMIGGLGLTLKKGKFKFF 3FG2 Chain:P ((1-310)) ----NDTVLIAGAGHAGFQVAVSLRQAKY--PGRIALINDEKHLPYQRPPLSKAYLKSGGDPNSLMFRPEKFFQDQAIELISDRMVSIDREGRKLLLASGTAIEYGHLVLATGARNRMLDVPNASLPDVLYLRTLDESEVLRQRM--------------PDKKHVVVIGAGFIGLEFAATARAKG-------------LEVDVVELAPRVMARVVTPEISSYFHDRHSGAGIRMHYGVRATEIAAEGDRVTGVVLSD----GNTLPCDLVVVGVGVIPNVEIAAAAGLPT-AAGIIVDQQLLT-SDPHISAIGDCALFESVRFGETMRVESVQNATDQARCVAARLTGD---------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3FG2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 1613 -25393 -15.74 -85.21 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain P : 0.68 3D Compatibility (PKB) : -15.74 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_3FG2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FG2-query.scw PDB file : Tito_Scwrl_3FG2.pdb: