Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEEKIYDVIIIGAGPAGMTAAVYTSRANLSTLMIERGIPGGQMANTEDVENYPGFESILGPELSNKMFEHAKKFGAEYAYGDIKEVID--GKEYKVVKAGSKEYKARAVIIAAGAEYKKIGVPGEKELGGRGVSYCAVCDGAFFKGKELVVVGGGDSAVEEGVYLTRFASKVTIVHRRDKLRAQSILQARAFDNEKVDFLWNKTVKEIHEENGKVGNVTLVDTVTGEESEFKTDGVFIYIGMLPLSKPFENLGI-TNEEGYIETNDRMETKVEGIFAAGDIREKSL--RQIVTATGDGSIAAQSVQHYVEELQETLKTLK 3F8P Chain:D ((16-318)) ------FDVIIVGLGPAAYGAALYSARYMLKTLVIGE-TPGGQLTEAGIVDDYLGLIEIQASDMIKVFNKHIEKYEVPVLL-DIVEKIENRGDEFVVKTKRKGEFKADSVILGIGVKRRKLGVPGEQEFAGRGISYCSVCDAPLFKNRVVAVIGGGDSALEGAEILSSYSTKVYLIHRRDTFKAQPIYVETVKKKPNVEFVLNSVVKEIKGDK-VVKQVVVENLKTGEIKELNVNGVFIEIGFDPPTDFAKSNGIETDTNGYIKVDEWMRTSVPGVFAAGDCTSAWLGFRQVITAVAQGAVAATSAYRYVTE---------

General information: TITO was launched using: RESULT: Template: 3F8P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1788 -26594 -14.87 -89.24 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain D : 0.81 3D Compatibility (PKB) : -14.87 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_3F8P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F8P-query.scw PDB file : Tito_Scwrl_3F8P.pdb: