Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQSIKLFSVLSDQFQNNPYAYFSQLREEDPVHYEES---IDSYFISRYHDVRYILQHPDIFTTKSLV--ERAEPVMRGPVLAQMHGKEHSAKRRIVVRSFIGDALDHLSPLIKQNAENLLAPYLE-RGKSDLVNDFGKTFAVCVTMDMLGLDKRDHEKISEWHSGVADFITSISQSPEARAHSLWCSEQLSQYLMPVIKERRVNPGSDLISILCTSEYEGMALSDKDILALILNVLLAATEPADKTLALMIYHLLNNPEQMNDVLADRSLVPRAIAETLRYKPPVQL-IPRQLSQDTVVGGMEIKKDTIVFCMIGAANRDPEAFEQPDVFNIHREDLGIKSAFSGAARHLAFGSGIHNCVGAAFAKNEIEIVANIVLDKMRNIRLEEDF--CYA-ESGLYTRGPVSLLVAFDGA 4MM0 Chain:A ((11-399)) ------HPYPFDRAVPTAIPPLYEELRETERVAAITMATGDPGFLVTRYEDVRFVLSDPR-FSVRQDLPGAPRLTEMTFESVMTTDPPVHTRLRRLLSRDFTARRIERMRPRLEEIAEGLLDEMEKKGAPADIVESLAVPFPITVICELLGVPMVDVARFRGWADTMVSLTG------YSMEDWTAARDALESYLDGLVAAKRENPGSDLLSALVATAAEDNELTDHDVRSLSLILLLAGYEPASNQLGSSVLTLLRFPDRLAELRRDPGLLPSAVEELMRYAPAGDGALFRVTLEDVTIGDTHIPANSAVLASTQAANWDPRRFDDPTGLRLDRPD----------NQHTALGHGIHFCLGAALARVELQVAIGALLRRFPRLALATDESGLRWSSPGSMLSGFAEIPVTW---

General information: TITO was launched using: RESULT: Template: 4MM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1869 146 0.08 0.38 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 0.08 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_4MM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MM0-query.scw PDB file : Tito_Scwrl_4MM0.pdb: