Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------MGESTSLKEI--LSTLTKRILLIMIVT-----AAATAAGGLISFFALTPIYENSTQILVNQSKNERKEVQFNDVQTNLQLINTYNVIIKSPAILDEVIKEMGLSMTSQ------------ELNDKITVSSEQDSQVVNISVRDE--NAETAAHIANTIASV--FQDKITSIMNVDNVSILSKAEVSEHPSPVSPK------------PLLNIAIAFAAGLAGSIGLAFLLEHLDNTIK---SEEQLESLLDIPVLGTVSTIANEQKTAKTLQGFQSEKTGSGHFGA---------- 2YYE Chain:A ((3-345)) GGIMVELLKLVRSSGCAAKVGPGDLQEILKGFNIYTDESTLVSIGDDAGVYEHNGIIWVYTVDIITPVVNDPYLWGAISTANALSDVYAMGGIPVNALAISCFNNCELDIEIFREVIRGALDKLREAKTVLLGGHTIDDKEPKFGLSVAGICPEGKYITQSGAQVGQLLILTKPIGTGILIKGLKEGILKEEDINEAIENMLALNDKARNLMLSLDATACTDVTGFGLLGHAWNICKNSNIGARIFFEKVPYYQLSENLVKKKIYPKGAIENLNFVKNYLKSNLDNWKLILLSDPVTSGGLLFTINKEKLEKIDETAKELEVNYWIIGETIAENVLEVL

General information: TITO was launched using: RESULT: Template: 2YYE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1159 36373 31.38 146.66 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 31.38 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.171

(partial model without unconserved sides chains): PDB file : Tito_2YYE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YYE-query.scw PDB file : Tito_Scwrl_2YYE.pdb: