Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLNKTAYELCMRMDGQKTAEQIL---AEQCAVYDESPEDHKDWYYDMLNMLQNKQVIQLGNRASRHTITTSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPEALGTVSIEQFKKTADMLFDNGVLTCEITGGEIFVHPNANEILDYVCKKFKKVAVLT-NGTLMRKESLELLKTYKQKIIVGISLDSVNSEVHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKVRDLGAKAFSYNWVDDFGRGRDIVHPTKDAEQHRKFMEYEQHVIDEFKDLIPIIPYERKRAANCGAGWKSIVISPFGEVRPCALFPKEFSLGNIFHDSYESIFNSPLVHKLWQAQAPRFSEHCMKDKCPFSGYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI----- 1A4M Chain:A ((1-349)) ------TPAFNKPKVELHVHLDGAIKPETILYFGKKRGIALPADTVEELRNIIGMDKPLSLPGFLAKFDYYMPVIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVDPMPWN-QTEGDVTPDDVVDLVNQGLQEGEQAFGI-------------------------------------------KVRSILCCMRHQPSWSLEVLELCKKYNQKTVVAMDLAG------DETIEGSSLFPGHVEAYEGAVKNGI--HRTVHAGEVG--SPEVVREAVDILKT--------ERVGHGYHTIED---------------------EALYNRLLKENMHFEVCPWSSYLTGAWDPKTTHAVVRFKNDKANYSLNTDDP-LIFKSTLDTDYQMTKKD--------------MGFTEEEFKRLNINAAKS----SFLPEEEKKELLERLYREYQ

General information: TITO was launched using: RESULT: Template: 1A4M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2049 19177 9.36 56.40 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 9.36 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.265

(partial model without unconserved sides chains): PDB file : Tito_1A4M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1A4M-query.scw PDB file : Tito_Scwrl_1A4M.pdb: