Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MKSLLAIYPGSIVSEELCIREGCLCFFDESNQRYISIPKTEISEKEVLVLQSFLTPADEGNQLSMKSPEENKWFSFLFSRGELPAYIKKRTRFVHFHLFGKIERTSFTEAVRHFWPVSFVIVWIHEDRGVIVEQESEAAAEKDELESLAKVLESDFYFSVRFYAGRFYEPDEC---LRKHYAREQAYFLFAEKRLPQVQSVTFEMIFPFLLLETEKEKLETLLSEEAELLFGSESELRKTIKLFIENNSNVTLTAKKLHLHRNSLQYRIDKFIERSGIDIKSYKGALLAYFICLQN--ESSE 2CVH Chain:A ((1-220)) MLSTGTKSLDSLLGGGFAPGVLTQVYG-----------------PYASGKTTLALQTGLLS---GKKVAYVDTEGG------FSPERLVQMAETR-------------GLNPEEALSRFILFTP----------------SDFKEQRRVIGSLKKTVDSNFALVVVDSITAHYRAEENRSGLIAELSRQLQVLLWIARKHNI----------PVIVINQVHFDSRTEMTKPV-----AEQTLGYRCKDILRLDKLPKPGLRVAVLERHRFR-----------------PEGLMAYFRITERGIEDVE

General information: TITO was launched using: RESULT: Template: 2CVH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1082 69097 63.86 329.03 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 63.86 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.222

(partial model without unconserved sides chains): PDB file : Tito_2CVH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CVH-query.scw PDB file : Tito_Scwrl_2CVH.pdb: