Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISKKVVLPLVFSAPFIFFFVLCIVVVMTISRENQVGDDFIGGGDGEYETVGIAPEVERFRAVFEKYARQEGVFDQVNIIMALT-MQESGGRSLDIM-QSSESIGLPPNSITDPERSIEVGIKHFKKVFKQAGG---DVRLTLQAYNFGSGFIDYVKKNGGKYTKKLALDFSRLQ------------AFKM---GWKSYGDPSYVDHVMRYVKGSDKNVKPVKGSMDFYETVM-KEALKYEGQPYAWGGSNPETGFDCSGLVQWSFAKAGITLPRTAQEQHGATKKIS-EKEATAGDLVFFGGTYE-GKAITHVGIYVGNGRMFNSNDSGIQYSDLKSGYWRDHLVSFGRIK-- 4FDY Chain:A ((46-340)) -------------------------------------------------GVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAI-MQVESGGTAED-VMQSSESLGLPPNSLSTEE-SIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYELAEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYL-----TDTSPTEFDDETVQVIMDEALKYEGFPYVFGGASPTT-SFDSGLIQWVYDKAGISLPRVAQDQYDATQEISME-EAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDP-IGYGDLSSRYWQDHLIGARRVIHN

General information: TITO was launched using: RESULT: Template: 4FDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1253 -121326 -96.83 -451.03 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -96.83 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_4FDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FDY-query.scw PDB file : Tito_Scwrl_4FDY.pdb: