Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSLKGKRIGFGLTGSHCTYEAVFPQIEELVNEGAEVRPVVTFNVKSTNTRFGEGAEWVKKIEDLTGY----EAIDSIV---KAEPLGPKLPLDCMVIAPLTGNSMSKLANAMTDSPVLMAAKATIRNNRPVVLGISTNDALGLNG---TNLMRLMSTKNIFFIPFGQDDPFKKPNSMVAKMDLLPQTIE-KALMHQQLQPILVENYQGND 2EJB Chain:A ((1-183)) -----MQKIALCITGASGVI-YGIKLLQVLEELDFSVDLVISRNAK----VVLK--------EEVLKGLKNVRIHEENDFTSPLASGSRLVHYRGVYVVPCSTNTLSCIANGINKNLIHRVGEVALKERVPLVLLV---REAPYNEIHLENMLKITRM-GGVVVPASP-AFYH----KPQSIDDMINFVVGKLLDVLRIEHNL--------

General information: TITO was launched using: RESULT: Template: 2EJB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 871 -125723 -144.34 -761.95 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -144.34 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.341

(partial model without unconserved sides chains): PDB file : Tito_2EJB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EJB-query.scw PDB file : Tito_Scwrl_2EJB.pdb: