Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTIKKDFIPVSNDNRPGYAMAPAYITVHNTANTAKGADAKMHAKFVKN--PNT-SESWHFTVDDSVIYQHLP-IDENGWHAGDGTN-GTGNRKSIGIEICENADG---DFEKATSNA-QWLIRKLMKENNIPLNR--VVPHKKWSGKECPRKLLDH--------------------WNSFLNGISSSDTPPKETSPSYPLPSGVIKLTSPYRKGTNILQLQKALAVLHFYPDKGAKNNGIDGVYGPKTANAVKRFQLMNGLTADGIYGPKTKAKLKSKLK 4OLS Chain:A ((28-212)) TLKVSKNHINYTMDK---RGKKPEGMVIHNDAGRSSGQQ-YEN-SLANAGYARYANGIAHYYGSEGYVWEAIDAKNQIAWHTGDGTGANSGNFRFAGIEVCQSMSASDAQFL-KNEQAVFQFTAEKFKEWGLTPNRKTVRLHMEFVPTACPHRSMVLHTGFNPVTQGRPSQAIMNKLKDYFIKQIKNYMDK------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OLS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 811 25264 31.15 164.05 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 31.15 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_4OLS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OLS-query.scw PDB file : Tito_Scwrl_4OLS.pdb: