Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKQIISWITDYQNTGDEAVLRQVREVCWPIVEAVLQEKVMDDEQANNLREKGIERFPFIISKYQADVQLPVETFLQNTYRFYFHQVMRESS 4MBQ Chain:A ((871-919)) ------DEAATKLDLARAYIDMGDSEGARDILDEVLAEG--NDSQQAEAREL-LERLA----------------------------------

General information: TITO was launched using: RESULT: Template: 4MBQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 155 -4243 -27.37 -86.59 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -27.37 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_4MBQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MBQ-query.scw PDB file : Tito_Scwrl_4MBQ.pdb: