Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHLPEQYRQLFPTLQTHTMLASCSQSALAEPVSRAIQDYYDSLLYKGTNWK---EAIEKTEFARNEFAKLIGAEPDEVAIVPSVSDALVSVASSLTAF------GKKHVVYTDMDFPAVPHVWQAHS---DYTVSVIPS-IDGVLPLEQYETHIS--DETVLTCVPHVHYRDGYVQDIKAIAEISQRKGSLLFVDAYQSAGHIPIDVKEWGVDMLAAGTRKYLLGIPGVAFLYVRKELADALKPKASAWFGRESGFDGAYAKVARRFQTGTPAFISVYAAAAALSLLNHIGVSHIRDHVKTICADAVQYAAEK--GLQLAAAQ-GGIQPGMVAIRDER--ASETAGLLKKKKVICAPR----------------ENVIRLAPHFYNTKEEMRHAIDEIAAKTIHK 4ISY Chain:A ((12-391)) -----------------SAYLDHAATTPMHPAAIEAMAAVQRTIGNASSLHTSGRSARRRIEEARELIADKLGARPSEVIFTAGGTESDNLAVKGIYWARRDAEPHRRRIVTTEVEHHAVLDSVNWLVEHEGAHVTWLPTAADGSVSATALREALQSHDDVALVSVMWANNEVGTILPIAEMSVVAMEFGVPMHSDAIQAVGQLPLDFGASGLSAMSVAGHKF-GGPPGVGALLLRRDVT--CVPLMHGGGQ------------ERDIRSGTPDVASAVGMATAAQIAV-DGLEENSARLRLLRDRLVEGVLAEIDDVCLNGADDPMRLAGNAHFTFRGCEGDALLMLLDANGIECSTGSPSHVLIAMGVDAASARGSLRLSLGHTSVEADVDAALEVLPGAVAR-

General information: TITO was launched using: RESULT: Template: 4ISY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2008 -224284 -111.70 -667.51 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -111.70 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_4ISY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ISY-query.scw PDB file : Tito_Scwrl_4ISY.pdb: