Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKLSEEMKQTIMDIFEHLHANPEVSWKEYETTSFLKQKLEDLGCRTRTFS---------------------DCTGVVGEIGS--GSPVVAVRADIDALWQEVNG-----------TFRANHSCGH---DSHMTMALGTLMLLKKQPELPKGTIRFIFQPAEEKGG-GALKMIEEGVLDDIDYLYGVHVRPIQETQNGRCAPSILHGSSQHIEGTIIGEEAHGARPHLGKNSIEIAAFLVHKLGLIHIDP---------Q----IPHTVKMTKLQAGGESSNIIPGKASFSLDLRAQTNEAMEALIAETERACEAAAAAF----GAKIELHKE-HSLPAATQ--NKEAEAIMAEAITEIIGAERLDDPLVTTGGEDFHFYA-V-KVPNLKTTMLGLGCGLQPGLHHPHMTFDRNAMFTGIHILANAVLKTFQKAESLAAANAS 3PFO Chain:A ((17-432)) LRAAVDRNFNDQVAFLQRMVQFRSVRGEEAPQQEWLAQQFADRGYKVDTFSLADVDIASHPKAAPMDTIDPAGSMQVVATADSDGKGRSLILQGHIDVVPEGPVDLWSDPPYEAKVRDGWMIGRGAQDMKGGVSAMIFALDAIRTAGYAPDARVHVQTVTEEESTGNGALSTLMRGY--RADACLIPEPT------GHT--LTRAQVGAVWFRLRVRGTPVHVAYSETGTSAILSAMHLIRAFEEYTKELNAQAVRDPWFGQVKNPIKFNVGIIKGGD-WASSTAAWCELDCRLGLLTGDTPQEAMRGIEKCLADAQATDSFLSENPAELVWSGFQADPAVCEPGGVAEDVLTAAHKAAFNAPL--DARLSTAVNDTRYYSVDYGIPA---LCYGPYG---QGPHAFDERIDLESLRKTTLSIALFVAEWCGLRK--------

General information: TITO was launched using: RESULT: Template: 3PFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2068 -165983 -80.26 -466.24 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -80.26 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_3PFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PFO-query.scw PDB file : Tito_Scwrl_3PFO.pdb: