Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MTVRYQIEQLGDSAMMIRFGEEINEQVNGIVHAAAAYIEEQPFPGFIECIPAFTSLTVFYDMYEVYKH-LPQGISSPFESVKRDVEERLAEIAEDYEVNRRIVEIPVCYGGEFGPDLEEVAKINQLSPEEVIDIHTNGEYVVYMLGFAPGFPFLGGMSKRIAAPRKSSPRPSIPAGSVGIAGLQTGVYPISTPGGWQLIGKTPLALFRPQENPPTLLRAGDIVKFVRISEKDYHAYKEESN------- 3B21 Chain:A ((21-212)) NVNVKKLLESLNSKSLGDMDKDSELAATLQKMIN-------------PSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQIDS------------------EGIVSHPNETYSKFRERIAENILQNTSKGS--VVMISIEQATHWIAGFNDG---------------EKIMFLDVQTG-------KGFNLYDPVE-------KSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC

General information: TITO was launched using: RESULT: Template: 3B21.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 860 -77114 -89.67 -433.22 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -89.67 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_3B21.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B21-query.scw PDB file : Tito_Scwrl_3B21.pdb: