Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGITKEEVNSYYQKAGIVLTDEEVDQIQLMDYGLGKERKVGLQLFVYVNTDRYCSKELVLFPGQTCPEHRHPPVDGQEGKQETFRCRYGKVYLYVEGEKTPLPKVLPPQEDREHYTVWHEIELEP--GGQYTIPPNTKHWFQAGEEGAVVTEMSSTSTDKHDIFTDPRI 1UPI Chain:A ((50-137)) --------------------------------------------------------CSVSSAGVLRGLHFAQL---PPSQAKYVTCVSGSVFDVVVDIRE----------GSPTFGRWDSVLLDDQDRRTIYVSEGLAHGFLALQDNSTVMYLCSAE------------

General information: TITO was launched using: RESULT: Template: 1UPI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 425 -25649 -60.35 -298.24 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -60.35 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_1UPI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UPI-query.scw PDB file : Tito_Scwrl_1UPI.pdb: