Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MYKVKVYVSLKESVLDPQGSAVQHALHSMTYNEVQDVRIGKYMELTIE-KSDRDLDVLVKEMCEKLLANTVIEDYRYEVEEVVAQ- 2N2T Chain:A ((1-84)) MGQWQIKIY-SENEREFRELIERLEEERPSVQYTETTR---NGRRQLTIRSNDKNEVDRILEEVRRKVPNARVRETETGSLEHHHHHH

General information: TITO was launched using: RESULT: Template: 2N2T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 274 -17059 -62.26 -213.23 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -62.26 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.335

(partial model without unconserved sides chains): PDB file : Tito_2N2T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2N2T-query.scw PDB file : Tito_Scwrl_2N2T.pdb: