Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIKRSFISISVLMVIFGLMISVQFNSLKHPKVRDTRDMWDIREELTSEQKKQEKLLAEINKYDKLLNSYSQTKEMTKETALNNTLQSLKKTAGMTDITGSGIVITISPLFSESLTGEPIENPPPDLLKKLINELNSYGAEHISINERRVVNHTVIRDINGTTKID-GYALDDYPLTVKVLAKDPDMLHSRVKGSGLEDLFASENLALKAGKSESKLTLKAYDRPLDVQQLKLLKD 2KW7 Chain:A ((1-157)) ----------------------------------------------MRVYKPEDVPNVQLADSTRLVTDEAGLLSNAQEEVMNGRLRAIRSS------HAVEFAVVTLP----SIGDAPLEDFTLKLARQW----------GVGNEKNNNGLLLVLVLDQRRVRFETGYGLEGYL---------PDGLLSRIIHDRMIPHFRSGNYAEGLSEGVLAVQQVLDGSLEHHHHHH----

General information: TITO was launched using: RESULT: Template: 2KW7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 799 -43239 -54.12 -277.17 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -54.12 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_2KW7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KW7-query.scw PDB file : Tito_Scwrl_2KW7.pdb: