Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTINIVAGGPKNLIPDLTGYTDEHTLWIGVDKGTVTLLDAGIIPVE-AFGDFDSITEQERRRIEKAAPALHVYQAEKDQTDLDLALDWALEK-QPDIIQIFGITGGRADHFLGN-IQLLYKGVKTNIK-IRLIDKQNHIQMFPPGEYDIEKDENKRYISFIPFSEDI-H-ELTLTGFKYPLNNCHITLGSTLCISNELIHSRGTFSFAKGILIMIRSTD 3CQ9 Chain:A ((3-213)) -TIVNLLVGGPTANYPAD--LTTIPGPWVGADRGALRLVKRGI-QPVMVVGDFTVKDALVG---------AIVVKPDQDHTDTQLAIKSIFEQLQPDEVHLYGATGGRLDHLLANMWLVLDPVFRQWAPQIKLIDKQNSVRFFLPGDYQITKEADKRYLAFVP----LMPMHLTLPDEKYQLDAAYNAYPIS-WASNEFSGNTGHFSFDAGVLAVIQSRD

General information: TITO was launched using: RESULT: Template: 3CQ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1005 -109200 -108.66 -557.14 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -108.66 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_3CQ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CQ9-query.scw PDB file : Tito_Scwrl_3CQ9.pdb: