Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MHITTKRLLIREF-EFKDWQAVYEYTSDSNVMKYIPEGVF-TEEDAKAFVNKNKGDNAEKFPVILRDEDCLIGHIVFYKYFGEHTYEIGWVFNPNYQNKGYASEAAQAILEYGFKEMNLHRIIATCQPENIPSYRVMKKIGMRREGFFKKCIPKGNEWWDEYYYAILEEEWN-------------------------------------------------------------------------------------------------------------------------- 2ZW5 Chain:A ((9-301)) TAHLRTARLELTPLDPAADARHLHHAYGDEEVMRWWTRPACADPAETERYLTSCAAAPGARLWTIRAPDGTVPGMAGLLG--GTDVPGLTWLLRRDSWGHGYATEAAAAVVGHALEDGGLDRVEAWIEAGNRRSLAVAARVGLTERARLAQHYPHRPGPHEMVVLGKARAEEPLTTLAVITELPVRDVAATLRLVEAALGARTAFAIGDPPEFAEAALTPWSAGPRFRLAAVPGPGPVEPVRLHLDAAGTADSLHRRAVDAGARVDGPPVRRPWGRSEFVITLPEGHELTVSAPV

General information: TITO was launched using: RESULT: Template: 2ZW5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 854 -99843 -116.91 -594.30 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -116.91 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_2ZW5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZW5-query.scw PDB file : Tito_Scwrl_2ZW5.pdb: