Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLYGVIGNPIGHSMSPDIHNASLKDLGLDGHYHAFKVEENDLEDAVKGIRALGVQGINVTVPHKVSIMDYLDHIDESAKVLGAVNTVRREGDKLVGYNTDGEGFVKSLMKVLDKPISELSFLMIGAGGAARAIFTTIVRNTPKKFDICNRTLEKAKRLTEATPSFHNKEVLSIKEAEERLEQYDVIIHTTSVGMY-PNVDDVPLSLQRAASSAVVCDIVYNPIQTALLKEASQKGLKTLDGVGMFVEQAALSFQLWTGQEPNIEKMRSIVIGKLGGTEC 2EV9 Chain:B ((4-262)) ----FAVLGHPVAHSLSPAMHAFALESLGLEGSYEAWDTPLEALPGRLKEVRR-AFRGVNLTLPLKEAALAHLDWVSPEAQRIGAVNTVLQVEGRLFGFNTDAPGFLEAL-KAGGIPLKGPALVLGAG--GAGRAVAFALREAGLEVWVWNRTPQRALALAE---EFGLRAVPLEKAREARL-----LVNATRVGLEDPSASPLPAELFPEEGAAV--DLVYRPLWTRFLREAKAKGLKVQTGLPMLAWQGALAFRLWTGLLPDPSGMEEAARRALG----

General information: TITO was launched using: RESULT: Template: 2EV9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1562 -96079 -61.51 -372.40 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -61.51 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_2EV9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EV9-query.scw PDB file : Tito_Scwrl_2EV9.pdb: