Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------MFLQP-LLAKKIIAEVKKM-------YEREVI-IV--NTDGLIMAGTNDERVGQFHEGALICAKERRSVIITK-EDETRLKGVKAGINL-PVFFD-H-DVIAVFGLTGEPAEIQPFGELLRKMTELFIKESRHLEQSQWRERMLE--S-FMIDWLQLKE-WSPSFLEKAQLLGVDLSSRRQMILIQGYEWS-PHDIEQMARSW-KSSYPADLFIRWGNER----ILINHEVPQHEQRDRLLRKILH--------ICSFANTASSQYTAAGAGRAVASSSLTDSYEQAEKALAVSLKRK-TPIFEEDLKLDMCLTEISPGTRNEFPQRVLGKALEHQELMNTIRTFFHHDLSLKQTAEDMHIHINTLR-YRL-AKAEQLTGLRFDRTEDVVTMYVALYFLDQDTK 1FUU Chain:B ((11-394)) QIQTNYDKVVYKFDDMELDENLLRGVFGYGFEEPSAIQQRAIMPIIEGHDVLAQAQSGTGKTGTFSIAALQRIDTSVKAPQALMLAPTRELALQIQKVVMALAFHMDIKVHACIG----GTSFVEDAEGLRDAQIVVGTPGRVFDNIQRRRFRTDKIKMFILD--EADEMLSSGFKEQIYQIFTLLPPTTQVVLLSA---TMPNDVLEVTTKFMRN--PVRILVKKDELTLEGIKQFYVNVEEEEYKYECLTDLYDSISVTQAVIFCNTRRKVEELTTKLRNDKFTVSAIYSDLPQQER------DTIMKEFRSGSSRILISTDLLARGIDVQQVSLVIN-----YDLPANKENYIHR---IGRGGKGVAINFVTNEDVGAMRELEKFYSTQIEELPSDIATLLN---------

General information: TITO was launched using: RESULT: Template: 1FUU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1585 49886 31.47 149.36 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 31.47 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.255

(partial model without unconserved sides chains): PDB file : Tito_1FUU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FUU-query.scw PDB file : Tito_Scwrl_1FUU.pdb: