Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVYHFVGIKGTGMSPLAQILHDNGYTVQGSDIEKFIFTQTALEKRNITILPFSAENIKPGMTVIAGNAFPDTHPEIEKAMSEGIPVIRYHKFLGDYMKKFTSVAVTGAHGKTSTTGLLAHVIQNAKPTSFLIGDGTG-QGN---E------NSEYFVFEACEYR-RH----FLSYQPDYAIMTNIDFDHPDYFSSIDDVFDAFQEMALQVNK--GIIACGDDEHLPKIHANV---PVVYYGTGEENDFQARNIVKSTEGTTFDVFVRNTFYDTFYIPAYGHHNVLNSLAVIALCHYEEIDSSIIKHALKSFGGVKRRFNEKQLGDQVLIDD-YAHHPTEIKVTIEAARQKYPDREIVAVFQPHTFT-RTQQFLDEFAESLS--GADCVYLCDIFGSARENAGKLTIGDLQGKIHNAKLI--EEDDTSVL-K-----AHDKAVLIFMGAGDIQKYMRAYENVMA 4QDI Chain:A ((120-461)) ----------------------------------------------------------------------------------------------------LKVIALTGSSGKTTTKEMLGSILSRLAP-TLITRGNLNNDLGVPMMLLELRKEHQYAVMELGANHQGEIDYTSKIVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYRHILPQGVAIVPQQDDFTAEIREAAKSHQIMSFGEG--GDVFATEIELLPQSANFQLHTP-QGSSFVRLPFAGEHNVQNATAAVAFALALGVSLEDIVKGLEQAQGAKGRLNFIQKAPHLFIDDTYNANPTSMRAAAQVLLQQN-GIKVMVMGDIGELGDSSWQEHHDLGRDLAELPLDHIVAVGQFA-SAA----LEGAGLH-ST-KLKAFQTQAEALPFLINLIQTHQPQSMSFLFKGSRFTHM----------

General information: TITO was launched using: RESULT: Template: 4QDI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1735 26700 15.39 86.13 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 15.39 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_4QDI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QDI-query.scw PDB file : Tito_Scwrl_4QDI.pdb: